Source: https://guthlabs.ai/docs/instruments/enzyme-inhibition-type-comparator/implementation/

instrument implementation / implementation-contract

# Enzyme Inhibition Type Comparator implementation

A source-bound implementation contract for Enzyme Inhibition Type Comparator; deployment and executable qualification remain separate.

Status: **VERIFIED_CLEAN**Lifecycle: **implementation-contract**

## Binding contract

This record describes what an executable surface must bind and validate. It does not claim that a public calculator, package, or production route is deployed.

**Record owner**: Guth Instruments

**Lifecycle**: verified-clean

**Evidence ID**: t6-059

**Effect**: Computation or source lookup only

## Input and validation requirements

The model has an undefined-result failure at Ki=0 with nonzero inhibitor concentration, and it also needs explicit validation for negative concentrations. Without validation, a negative inhibitor concentration can yield a mathematically valid but physically inappropriate result.

## Required corrections

No binding erratum is attached to this unique-name record. Implementers must still preserve the declared units, domain, and failure behavior.

## Inspectable interface fixture

The sample below is evidence for interface and validation tests, not a production implementation.

````
{
  "inputs": {
    "Vmax": 100,
    "Km": 10,
    "Ki": 5,
    "I": 5,
    "inhibition_type": "competitive",
    "substrate_concentrations": [
      0,
      10,
      100
    ]
  },
  "expected_outputs": {
    "alpha": 2,
    "apparent_Vmax": 100,
    "apparent_Km": 20,
    "inhibited_velocity": [
      0,
      33.3333333333,
      83.3333333333
    ],
    "uninhibited_velocity": [
      0,
      50,
      90.9090909091
    ],
    "curve_features": {
      "inhibited_root": [
        0
      ],
      "uninhibited_root": [
        0
      ],
      "inhibited_horizontal_asymptote": 100,
      "uninhibited_horizontal_asymptote": 100,
      "inhibited_extrema": "none for S>=0",
      "uninhibited_extrema": "none for S>=0"
    }
  },
  "working": "alpha=1+[I]/Ki=1+5/5=2. Competitive equation: v=100S/(2*10+S)=100S/(20+S). At S=0: v=100*0/(20+0)=0. At S=10: v=100*10/(20+10)=1000/30=33.3333333333. At S=100: v=100*100/(20+100)=10000/120=83.3333333333. Apparent parameters: Vmax,app=100 and Km,app=alpha*Km=2*10=20. Uninhibited equation: v0=100S/(10+S). At S=0: 0. At S=10: 1000/20=50. At S=100: 10000/110=90.9090909091. The root is S=0 and the horizontal asymptote is Vmax=100."
}
````

## Other records

[01**Overview**](<https://guthlabs.ai/docs/instruments/enzyme-inhibition-type-comparator/>)[02**Mechanism**](<https://guthlabs.ai/docs/instruments/enzyme-inhibition-type-comparator/mechanism/>)[04**Qualification**](<https://guthlabs.ai/docs/instruments/enzyme-inhibition-type-comparator/qualification/>)

## Sources and evidence

These pointers bind the page to evidence. They do not grant authority or collapse verification into deployment.

- `VERIFIED_LEDGER.json`

- `evidence-tranche6/tranche6-results.json`