Source: https://guthlabs.ai/docs/instruments/pcr-cycle-amplification-calculator/implementation/

instrument implementation / implementation-contract

# PCR Cycle / Amplification Calculator implementation

A source-bound implementation contract for PCR Cycle / Amplification Calculator; deployment and executable qualification remain separate.

Status: **VERIFIED_CLEAN**Lifecycle: **implementation-contract**

## Binding contract

This record describes what an executable surface must bind and validate. It does not claim that a public calculator, package, or production route is deployed.

**Record owner**: Guth Instruments

**Lifecycle**: verified-clean

**Evidence ID**: t4-098

**Effect**: Computation or source lookup only

## Input and validation requirements

An efficiency of -2 with a fractional cycle count of 0.5 makes the amplification base negative and raises it to a non-integer power, producing no real-valued result. The calculator should reject this input or explicitly return undefined rather than silently returning an invalid quantity.

## Required corrections

No binding erratum is attached to this unique-name record. Implementers must still preserve the declared units, domain, and failure behavior.

## Inspectable interface fixture

The sample below is evidence for interface and validation tests, not a production implementation.

````
{
  "inputs": {
    "mode": "amplification",
    "initial_copies": 1000,
    "efficiency": 1,
    "cycles": 10
  },
  "expected_outputs": {
    "final_copy_number": 1024000,
    "fold_amplification": 1024
  },
  "working": "1. Convert efficiency: E=1, so 1+E=2. 2. Compute fold amplification: 2^10=1024. 3. Compute final copies: 1000×1024=1,024,000."
}
````

## Other records

[01**Overview**](<https://guthlabs.ai/docs/instruments/pcr-cycle-amplification-calculator/>)[02**Mechanism**](<https://guthlabs.ai/docs/instruments/pcr-cycle-amplification-calculator/mechanism/>)[04**Qualification**](<https://guthlabs.ai/docs/instruments/pcr-cycle-amplification-calculator/qualification/>)

## Sources and evidence

These pointers bind the page to evidence. They do not grant authority or collapse verification into deployment.

- `VERIFIED_LEDGER.json`

- `evidence-tranche4/tranche4-results.json`