ng-to-pmol / Copy Number Converter implementation

Binding contract

This record describes what an executable surface must bind and validate. It does not claim that a public calculator, package, or production route is deployed.

Record owner

Guth Instruments

Lifecycle

verified-clean

Evidence ID

t4-110

Effect

Computation or source lookup only

Input and validation requirements

The supplied pmol formula is dimensionally inconsistent with mass in ng and molecular weight in g/mol: for 650 ng of a 1000-bp dsDNA molecule it returns 1000 pmol, while the copy-number formula gives 6.022×10^11 copies, corresponding to 1 pmol. The instrument also needs rejection handling for zero length, unsupported molecule types, and negative mass.

Required corrections

No binding erratum is attached to this unique-name record. Implementers must still preserve the declared units, domain, and failure behavior.

Inspectable interface fixture

The sample below is evidence for interface and validation tests, not a production implementation.

{
  "inputs": {
    "mass_ng": 1,
    "length_bp": 1000,
    "MW_g_per_mol": 650000,
    "molecule_type": "dsDNA"
  },
  "expected_outputs": {
    "pmol": 0.00153846153846,
    "copies": 926483193.846154
  },
  "working": "MW=650×1000=650000 g/mol. pmol=(1 ng×1000)/650000=1000/650000=0.00153846153846 pmol. mass_g=1×10^-9=0.000000001 g. copies=(0.000000001/650000)×6.02214076×10^23=1.53846153846×10^-15×6.02214076×10^23=9.26483193846×10^8=926483193.846154 copies."
}

Other records

Sources and evidence

These pointers bind the page to evidence. They do not grant authority or collapse verification into deployment.

• VERIFIED_LEDGER.json
• evidence-tranche4/tranche4-results.json